2012: Development of PCR Multiplexed Canine Marker Panels for the Purposes of Genome Screening and Linkage Analysis
Grant Status: Closed
Abstract
A number of tests have been developed to screen for affected or carrier animals with genetic diseases. The development of many of these tests was possible due to the availability of information regarding similar disorders in humans. The majority of disorders affecting purebred dogs have no known counterpart in humans and therefore no candidate gene. However, an indirect test that screens for a strong association of a microsatellite DNA marker with a disease phenotype can be incorporated in a simple diagnostic test for carriers of the trait. The ultimate goal of the research is to design marker panels containing a minimum of 300 microsatellite markers covering all the linkage groups or chromosomes of the canine genome. The Veterinary Genetics Laboratory has completed panels containing 100 markers with known map locations. This project will incorporate at least 200 additional markers that will then represent the entire canine genome and allow effective screening for linkage of markers to disease gene(s).
Publication(s)
Eggleston, M. L., Irion, D. N., Schaffer, A. L., Hughes, S. S., Draper, J. E., Robertson, K. R., … Pedersen, N. C. (2002). PCR multiplexed microsatellite panels to expedite canine genetic disease linkage analysis. Animal Biotechnology, 13(2), 223–235. https://doi.org/10.1081/ABIO-120016191
Help Future Generations of Dogs
Participate in canine health research by providing samples or by enrolling in a clinical trial. Samples are needed from healthy dogs and dogs affected by specific diseases.
