2003: Anchoring the Canine Map Through BAC Mapping
Grant Status: Closed
AbstractGenome maps are essential in identifying genes that cause inherited disease. They consist of a series of markers, positioned along each chromosome, which act as reference points for navigating different regions of the genome. Currently, the canine map is composed of about several hundred such markers, which provide "addresses" for over 90 percent of the genome. This early version of the map has proven useful for identifying the general location of several disease genes. But a much more highly refined map is necessary if we are to actually clone disease genes of interest (not just identify their location) and, subsequently, develop highly reproducible genetic tests. This proposal aims to characterize several hundred random clones, each containing a small portion of the canine genome, and then mapping them on the existing map relative to the markers and genes already in place. These clones, called BACs, will serve as "entry points" along the canine genome. Once a region of the genome is identified where a disease gene lies, the BAC clones will serve as starting points for investigators to genetically "walk" up and down the region and eventually clone the gene of interest. The work done as a result of this proposal is not breed specific, rather it will benefit all breeds of dogs equally.
Help Future Generations of Dogs
Participate in canine health research by providing samples or by enrolling in a clinical trial. Samples are needed from healthy dogs and dogs affected by specific diseases.