03017: Molecular Characterization of Canine Soft Tissue Sarcomas Using Spatially Defined Proteomics and Transcriptomics

Grant Status: Open

Grant Amount: $50,841
Enni Markkanen, Dr. med. vet., Dr. sc. nat.; University of Zurich
March 1, 2022 - August 31, 2023

Sponsor(s):

Breed(s): -All Dogs
Research Program Area: Oncology
Donate to Support this Research Program Area

One Health: Yes

Abstract

Soft-tissue sarcomas (STS) are frequent cancers that affect dogs of all breeds and can occur almost anywhere in the body. Therapy usually consists of surgical removal of these tumors, but it is difficult for the surgeon to distinguish the exact borders of the tumor, which often leads to incomplete removal and regrowth of the tumor in many affected patients. Novel approaches, such as specific anticancer drugs or tumor-cell-specific dyes to improve visualization during surgery have great potential to improve care for STS patients. However, the development of such approaches for STS is frustrated by a striking lack of molecular data to illuminate what mechanisms drive the growth of these tumors or to define targets that specifically differentiate tumor cells from the normal peritumoral tissue (PTT). This lack of knowledge on an extremely relevant canine tumor type warrants further investigation.

Using an innovative approach to analyze specific areas of archival patient samples by laser-capture microdissection (LCM), RNAsequencing, and proteomics, the overarching goal of this project is to gain a detailed molecular understanding of the very frequent STS subtypes: perivascular wall tumors (PWT) and peripheral nerve sheath tumors (PNST). This information will complement preliminary data for fibrosarcoma (FSA) and may enable development of future novel diagnostic and therapeutic modalities for patients, including identification of specific anticancer drugs and targeted tumor visualization strategies to guide surgical excision of STS. As complete tumor removal with clean margins is the most important factor to influence recurrence, metastasis, and survival in patients, precise visualization of STS has tremendous potential to improve the currently available options to treat these canine cancers. The knowledge gained through the study will work to improve the care and therapy of dogs with STS. Additionally, as canine STS are considered good models to better understand STS in humans, these results also have the potential to significantly impact human health from a One Health perspective.

Publication(s)

None at this time.

Help Future Generations of Dogs

Participate in canine health research by providing samples or by enrolling in a clinical trial. Samples are needed from healthy dogs and dogs affected by specific diseases.

Learn How to Help

Get Canine Health News:
Please leave this field empty
American Kennel Club Canine Health Foundation, Inc

8051 Arco Corporate Dr.
Suite 300
Raleigh, NC 27617
(888)-682-9696

Tax ID# 13-3813813

   2020 GuideStar logo

© 2021 AKC Canine Health Foundation | Privacy Policy | Site Map

Site by Blackbaud, Inc.