662: A Canine Linkage Map with More Than 2000 New Markers Mined from the Genome Sequence

Grant Status: Closed

Grant Amount: $299,000.16
Mark W. Neff, PhD; University of California, Davis
July 1, 2005 - December 31, 2006


Breed(s): -All Dogs
Research Program Area: General Canine Health
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The Broad Institute at MIT/Harvard has made public a whole-genome sequence of the dog. This resource renders obsolete most preceding canine genomic maps, with the exception of the linkage map, which reflects differential rates of recombination across the dog genome that must be derived empirically. Whereas the genome sequence provides the most powerful platform for interrogating and cloning individual genes, the linkage map is the principal tool for gaining initial genetic access to complex disease pathways. Despite its fundamental importance, the current version of the linkage map consists of less than 500 markers (in contrast, the human map has ~ 15,000 loci and 30-fold greater resolution). A comprehensive linkage map is an important unmet need in canine genetics. Here we outline a plan to effectively complete the canine map by developing ~ 2700 evenly spaced loci from the sequence, and typing these markers on several dog families to generate a robust dataset for linkage. Rigorous analysis of these data will produce an accurate map that allows researchers to fully explore the genetic landscape of the dog genome for genes of biological interest and practical importance. Given that the new map will be a catalyst for discovery, we will publish several refinements on the Internet prior to publication in a scientific journal.


Greer, K. A., Wong, A. K., Liu, H., Famula, T. R., Pedersen, N. C., Ruhe, A., … Neff, M. W. (2010). Necrotizing meningoencephalitis of Pug Dogs associates with dog leukocyte antigen class II and resembles acute variant forms of multiple sclerosis. Tissue Antigens. https://doi.org/10.1111/j.1399-0039.2010.01484.x
Wong, A. K., & Neff, M. W. (2009). DOGSET?: pre-designed primer sets for fine-scale mapping and DNA sequence interrogation in the dog. Animal Genetics, 40(4), 569–571. https://doi.org/10.1111/j.1365-2052.2009.01875.x
Wong, A. K., Ruhe, A. L., Dumont, B. L., Robertson, K. R., Guerrero, G., Shull, S. M., … Neff, M. W. (2010). A Comprehensive Linkage Map of the Dog Genome. Genetics, 184(2), 595–605. https://doi.org/10.1534/genetics.109.106831

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