00570-A: Capping Chromosome Ends with de Novo Genetic Markers to Ensure Closure of the Canine Linkage Map
Grant Status: Closed
Grant Amount: $12,960
Mark W. Neff, PhD; University of California, Davis
January 1, 2005 - December 31, 2005
Sponsor(s): Borzoi Club of America, Forsyth Kennel Club, Golden Retriever Foundation, Great Pyrenees Club of America, Great Pyrenees Club of Puget Sound, Greyhound Club of America, Irish Setter Club of America Foundation, Irish Wolfhound Club of America, Inc., Newfoundland Club of America Charitable Trust, Rhodesian Ridgeback Club of the United States, Rottweiler Health Foundation, St. Bernard Club of America, Starlight Fund
Breed(s): -All Dogs
Research Program Area: General Canine Health
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Abstract
There are several types of genetic maps, and most are made obsolete by a complete genome sequence. The linkage map is the lone exception; this map is an abstract representation of the genome, and thus cannot be directly inferred from the sequence. The current version of the linkage map is incomplete, and composed of less than 300 markers. This density is inadequate for powerful mapping approaches that exploit the genetic structure of breeds, and hold enormous promise for resolving complex canine diseases. Thus, a dense linkage map is of great importance to the research community. "Capping" the ends of chromosomes with informative DNA markers leveraged from the genome sequence represents a key first step in closing the dog linkage map. This approach extends the map to the end of every chromosome, thereby allowing researchers to explore the full genetic landscape for genes of practical importance. "Capping" the linkage map has immediate applications for ongoing studies � project-specific estimates of map coverage can be made, which allow researchers to assess how well their families and markers are performing at each stage of a project, and make adjustments accordingly.Publication(s)
Wong, A. K., & Neff, M. W. (2009). DOGSET?: pre-designed primer sets for fine-scale mapping and DNA sequence interrogation in the dog. Animal Genetics, 40(4), 569–571. https://doi.org/10.1111/j.1365-2052.2009.01875.x
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