0001808B: An Integrated Linkage and Radiation Hybrid Map of the Dog: A Collaborative Project

Grant Status: Closed

Grant Amount: $150,000
Francis Galibert, PhD; CNRS - University of Rennes
November 24, 1999 - November 23, 2001


Breed(s): -All Dogs
Research Program Area: General Canine Health
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Genome maps or organisms are essential for locating and identifying genes that cause inherited disease. They consist of a series of markers, positioned along each chromosome, which act as reference points or address labels for different regions of the genome. Currently, the canine map is composed of about 400 such markers, which, in principal, provides "addresses" for about 75 percent of the genome. This early version of the map has proven useful for identifying the general location of disease genes in several breeds of dog. But a much more highly refined map is necessary if we are to have the ability to actually identify disease genes (not just their location) and to develop highly reproducible genetic tests for canine diseases. In addition, the current version of the map allows us to navigate around the genome only in relatively outbred pedigrees of dog. Many disease genes of interest are found in pedigrees or breeds where a limited gene pool has forced significant levels of inbreeding. A much higher resolution map with many additional markers is necessary to map disease genes within inbred families. This proposal aims to identify numerous additional markers and place them on the canine genome map. In addition, it aims to map many more genes on the map. Finally, it will link the evolving canine map to the more well developed maps of the human and mouse genomes. The resulting map will be useful for the identification of disease genes in all breeds of dog, and should allow canine geneticists to increase the rate at which they identify genes responsible for inherited diseases in the dog.


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Hitte, C. (2003). Comparison of MultiMap and TSP/CONCORDE for Constructing Radiation Hybrid Maps. Journal of Heredity, 94(1), 9–13. https://doi.org/10.1093/jhered/esg012
Hitte, Christophe, Kirkness, E. F., Ostrander, E. A., & Galibert, F. (2008). Survey Sequencing and Radiation Hybrid Mapping to Construct Comparative Maps. Phylogenomics. Methods in Molecular Biology, 422, 65–77. https://doi.org/10.1007/978-1-59745-581-7_5
Hitte, Christophe, Madeoy, J., Kirkness, E. F., Priat, C., Lorentzen, T. D., Senger, F., … Galibert, F. (2005). Facilitating genome navigation: survey sequencing and dense radiation-hybrid gene mapping. Nature Reviews Genetics, 6(8), 643–648. https://doi.org/10.1038/nrg1658
Lowe, J. K., Guyon, R., Cox, M. L., Mitchell, D. C., Lonkar, A. L., Lingaas, F., … Murphy, K. E. (2003). Radiation hybrid mapping of the canine type I and type IV collagen gene subfamilies. Functional & Integrative Genomics, 3(3), 112–116. https://doi.org/10.1007/s10142-003-0082-x
Miller, A. B., Lowe, J. K., Ostrander, E. A., Galibert, F., & Murphy, K. E. (2001). Cloning, sequence analysis and radiation hybrid mapping of a mammalian KRT 2p gene. Functional & Integrative Genomics, 1(5), 305–311. https://doi.org/10.1007/s101420100038
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Quignon, P., Kirkness, E., Cadieu, E., Touleimat, N., Guyon, R., Renier, C., … Galibert, F. (2003). Comparison of the canine and human olfactory receptor gene repertoires. Genome Biology, 4(12), R80. https://doi.org/DOI:    10.1186/gb-2003-4-12-r80
Richman, M., Mellersh, C. S., André, C., Galibert, F., & Ostrander, E. A. (2001). Characterization of a minimal screening set of 172 microsatellite markers for genome-wide screens of the canine genome. Journal of Biochemical and Biophysical Methods, 47(1–2), 137–149. https://doi.org/10.1016/S0165-022X(00)00160-3
Sargan, D. R., Aguirre-Hernandez, J., Galibert, F., & Ostrander, E. A. (2007). An Extended Microsatellite Set for Linkage Mapping in the Domestic Dog. Journal of Heredity, 98(3), 221–231. https://doi.org/10.1093/jhered/esm006

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