03143: Causative Role of Xenobiotic Exposures in Canine Gallbladder Mucocele Formation

Grant Status: Open

Grant Amount: $37,172
Jody Gookin, DVM, PhD; North Carolina State University
February 1, 2023 - January 31, 2025


Breed(s): Shetland Sheepdog, -All Dogs
Research Program Area: Hepatic Disease
Donate to Support this Research Program Area

One Health: Yes


Mucocele formation is the most common gallbladder disease to afflict dogs. Despite the best surgical care, 17% will not survive. Retrospective studies alone report numbers of affected dogs in the thousands and over 80 publications have now addressed this specific disease. We don’t know what causes mucocele formation and have no way to predict, prevent, or reverse the epidemic rise in suffering and lost lives of these dogs. The long-range goal of our research is to identify the cause of mucocele formation in dogs. The objective of this study is to investigate a specific xenobiotic exposure that we identified in dogs with mucocele formation, for its potential to be the underlying cause of this disease. The hypothesis is that the discovered compound will be sufficient to inhibit fluid secretion and promote abnormal mucus formation by canine gallbladder epithelial cells; mechanisms that are consistent with an ability to promote mucocele formation in dogs. To test this hypothesis, investigators developed methods for culture of 3D “mini-gallbladder” organoids from a single canine gallbladder for use as a model for studying mucocele formation. This proposal aims are to determine if the discovered compound is sufficient to inhibit fluid secretion and/or promote accumulation of abnormal mucus secretions by canine gallbladder organoids. If the discovered compound mediates these mucocele-promoting effects, these studies will have identified the most probable cause of mucocele formation in dogs. This outcome is expected to have a significant impact on a prevalent problem by enabling veterinarians to prevent and treat this disease. 


None at this time.

Help Future Generations of Dogs

Participate in canine health research by providing samples or by enrolling in a clinical trial. Samples are needed from healthy dogs and dogs affected by specific diseases.

Learn How to Help

Get Canine Health News:
Please leave this field empty
American Kennel Club Canine Health Foundation, Inc

8051 Arco Corporate Dr.
Suite 300
Raleigh, NC 27617

Tax ID# 13-3813813


© 2021 AKC Canine Health Foundation | Privacy Policy | Site Map

Site by Blackbaud, Inc.