02838: Standardization of rLiNTPDase2 and Derived Chimeras as Antigens on Immunochromatographic Assay for Diagnosis of Canine Visceral Leishmaniasis

Grant Status: Closed

Grant Amount: $41,040
Juliana Fietto, PhD; Federal University of Viçosa
April 1, 2021 - September 30, 2023

Sponsor(s): Golden Retriever Foundation®

Breed(s): -All Dogs
Research Program Area: Immunology and Infectious Disease
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One Health: Yes


Canine visceral leishmaniasis (CVL) corresponds to the most aggressive and lethal form of leishmaniasis manifesting in dogs. In North, Central and South America, CVL is caused by Leishmania infantum and diagnostic techniques currently used to identify infected animals have important limitations in sensitivity and specificity. The main goal of this study is to develop new and accessible technologies for CVL diagnosis based on more specific antigens. This accomplishment has high potential to create a positive impact on diagnostic strategies all over the globe and to increase the amount of correctly diagnosed and treated dogs.


de Castro, R. B., de Souza, A. C. A., Pavione, N. da R. T., de Moraes, J. V. B., Ribeiro, I. C., de Melo Agripino, J., Bressan, G. C., de Souza Vasconcellos, R., Silva-Júnior, A., & Fietto, J. L. R. (2022). Urea, salts, and Tween 20 influence on adsorption of IgG and Leishmania rNTPDase2 to nitrocellulose. Analytical Biochemistry, 114648. https://doi.org/10.1016/j.ab.2022.114648

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