02838: Standardization of rLiNTPDase2 and Derived Chimeras as Antigens on Immunochromatographic Assay for Diagnosis of Canine Visceral Leishmaniasis
Grant Status: Closed
One Health: Yes
Abstract
Canine visceral leishmaniasis (CVL) corresponds to the most aggressive and lethal form of leishmaniasis manifesting in dogs. In North, Central and South America, CVL is caused by Leishmania infantum and diagnostic techniques currently used to identify infected animals have important limitations in sensitivity and specificity. The main goal of this study is to develop new and accessible technologies for CVL diagnosis based on more specific antigens. This accomplishment has high potential to create a positive impact on diagnostic strategies all over the globe and to increase the amount of correctly diagnosed and treated dogs.
Publication(s)
Castro RB, Badaró de Moraes JV, de Souza ACA, Favarato ES, Voorwald FA, Dos Santos FM, Bressan GC, Vasconcellos RS, Fietto JLR. High-resolution mapping of linear epitopes from LiNTPDase2: Advancing leishmaniasis detection using optimized protein and peptide antigens. Diagn Microbiol Infect Dis. 2024 Jul 18;110(2):116448. doi: 10.1016/j.diagmicrobio.2024.116448. Epub ahead of print. PMID: 39029390.
de Castro, R. B., de Souza, A. C. A., Pavione, N. da R. T., de Moraes, J. V. B., Ribeiro, I. C., de Melo Agripino, J., Bressan, G. C., de Souza Vasconcellos, R., Silva-Júnior, A., & Fietto, J. L. R. (2022). Urea, salts, and Tween 20 influence on adsorption of IgG and Leishmania rNTPDase2 to nitrocellulose. Analytical Biochemistry, 114648. https://doi.org/10.1016/j.ab.2022.114648
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