02553: Targeted Next Generation Sequencing Panel for Comprehensive Testing of Vector-borne Pathogens

Grant Status: Closed

Grant Amount: $103,245
Rebecca Wilkes, DVM; Purdue University
February 1, 2019 - July 31, 2021

Sponsor(s): American Chesapeake Club, Inc., Australian Terrier International, Boston Terrier Club of America, Gordon Setter Club of America, Irish Setter Club of America Foundation, Inc., Labrador Retriever Club, Inc., Papillon Club of America, Portuguese Water Dog Foundation, Inc., Rhodesian Ridgeback Charitable Foundation, Samoyed Club of America Education & Research Foundation

Breed(s): -All Dogs
Research Program Area: Tick-Borne Disease Initiative
Donate to Support this Research Program Area

One Health: Yes


Diagnosing vector-borne disease (VBD) in dogs can be difficult for a number of reasons. First, there are many different disease-causing agents that can be transmitted from ticks/fleas, and the clinical signs caused by these agents in dogs can overlap. Additionally, because ticks/fleas can harbor more than one agent at a time, multiple pathogens may be passed to a dog with a single vector bite, resulting in co-infections. VBD infections can initially present with non-specific signs, such as fever, lethargy, vomiting, diarrhea, and/or respiratory signs. Severe cases can be associated with neurologic signs. These signs can be a diagnostic conundrum. While initial blood work can be helpful and suggest VBD, it does not determine the infecting agent. This study will develop a comprehensive next generation sequencing panel to detect and identify major VBD agents known to cause disease in dogs and to aid in diagnosis of active infections. Additionally, through parallel sequencing with this method, this panel will incorporate testing for additional infectious diseases that may cause GI, respiratory, or neurologic signs in dogs. The comprehensive nature of this sequencing panel should be a useful tool for surveillance of infectious diseases in the canine population for rapid identification of VBD in dogs and protection of pet owners from such zoonotic diseases.


Kattoor, J. J., Nikolai, E., Qurollo, B., & Wilkes, R. P. (2022). Targeted Next-Generation Sequencing for Comprehensive Testing for Selected Vector-Borne Pathogens in Canines. Pathogens, 11(9), 964. https://doi.org/10.3390/pathogens11090964

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