01421: Genomic Resources for the Control of Canine Pyoderma
Grant Status: Closed
Project Summary
The emergence of methicillin-resistant staphylococci has made the treatment of canine skin infections with Staphylococcus pseudintermedius a difficult challenge. In fact some of these bacteria are resistant to all available antibiotics. There are many unanswered questions regarding antibiotic resistance and the underlying causes of skin infections. In addition, new strategies are needed to prevent and/or treat infections from these organisms such as vaccines or bacteriophage therapy. The foundation of these approaches rests on identification of the major strains of the bacterial species. To attain this goal we developed a new method of genetic tying and hundreds of bacterial isolates were obtained from all regions of the United States. Each sample was studied with regard to spectrum of antibiotic resistance and genetic background and we have made significant progress toward identifying the entire genome of the major strains of the bacterium. Using the latest technology, genomes have been assembled from two isolates representing the major clonal populations of S.pseudintermedius. We have nearly completed assembling the genomes of three additional genomes and plan to assemble a total of seven genomes. This project has provided important new information by identifying all of the thousands of genes contained within two isolates representing the most frequently occurring type of the bacterium. This information is being used to determine the role of the gene products in infection, to identify new targets for vaccine development, and to better understand the spread of antibiotic resistance between bacteria.
Publication(s)
Moodley, A., Riley, M. C., Kania, S. A., & Guardabassi, L. (2013). Genome Sequence of Staphylococcus pseudintermedius Strain E140, an ST71 European-Associated Methicillin-Resistant Isolate. Genome Announcements, 1(2). https://doi.org/10.1128/genomeA.00207-12
Riley, M. C., Perreten, V., Bemis, D. A., & Kania, S. A. (2016). Complete Genome Sequences of Three Important Methicillin-Resistant Clinical Isolates of Staphylococcus pseudintermedius. Genome Announcements, 4(5). https://doi.org/10.1128/genomeA.01194-16
Solyman, S. M., Black, C. C., Duim, B., Perreten, V., van Duijkeren, E., Wagenaar, J. A., … Kania, S. A. (2013). Multilocus Sequence Typing for Characterization of Staphylococcus pseudintermedius. Journal of Clinical Microbiology, 51(1), 306–310. https://doi.org/10.1128/JCM.02421-12
Videla, R., Solyman, S. M., Brahmbhatt, A., Sadeghi, L., Bemis, D. A., & Kania, S. A. (2018). Clonal Complexes and Antimicrobial Susceptibility Profiles of Staphylococcus pseudintermedius Isolates from Dogs in the United States. Microbial Drug Resistance, 24(1), 83–88. https://doi.org/10.1089/mdr.2016.0250
Help Future Generations of Dogs
Participate in canine health research by providing samples or by enrolling in a clinical trial. Samples are needed from healthy dogs and dogs affected by specific diseases.
