00270-A: In Vitro Immunosuppressive Effects of Metronidazole on Canine Lymphocytes

Grant Status: Closed

Grant Amount: $12,096
Albert E. Jergens, DVM, PhD; Iowa State University
February 1, 2005 - January 30, 2006


Breed(s): -All Dogs
Research Program Area: Gastrointestinal Disease
Donate to Support this Research Program Area


Immunosuppressive drugs are commonly administered to human and veterinary patients to control immune-mediated inflammatory diseases. Administration of a combination of immunomodulatory drugs with different mechanisms of action should result in more effective immunosuppression and fewer drug-induced adverse effects. Anecdotal evidence supports the use of corticosteroids, sulfasalazine, and metranidazole in the therapy of human and canine inflammatory bowel disease (IBD). Corticosteroids and sulfasalazine exert nonspecific immunomodulatory actions in IBD patients to inhibit cellular infiltration and the formation and function of various pro-inflammatory mediators. Metronidazole has been hypothesized to inhibit cellular immunity (and hence mucosal inflammation seen with IBD) although conclusive studies attesting to its immunomodulatory effects have not been performed. We propose to investigate the immunomodulating effects of metronidazole on mitogen-stimulaed proliferation of lymphocytes in blood collected from healthy dogs. Qualitative and quantitative evaluation of canine lymphocytes sub-population proliferation will be performed using two- color flow cytometric analysis. Because clonal expansion of lymphocytes is essential for antigen-induced immune responses, the ability of metronidazole to inhibit mitogen-stimulated lymphocyte proliferation provides an in vitro assessment of the effect of this drug on immune function. The results from this study will provide a rational basis for using metronidazole in immunosuppressive drug regimens for treatment of canine IBD.


None at this time.

Help Future Generations of Dogs

Participate in canine health research by providing samples or by enrolling in a clinical trial. Samples are needed from healthy dogs and dogs affected by specific diseases.

Learn How to Help

Get Canine Health News:
Please leave this field empty
American Kennel Club Canine Health Foundation, Inc

8051 Arco Corporate Dr.
Suite 300
Raleigh, NC 27617

Tax ID# 13-3813813


© 2021 AKC Canine Health Foundation | Privacy Policy | Site Map

Site by Blackbaud, Inc.