02535-MOU: Clinical and Molecular Genetic Analysis of Juvenile-Onset Laryngeal Paralysis in American Staffordshire Terriers
Grant Status: Open
We will investigate of a newly recognized, fatal, neurologic disease referred to as American Staffordshire Terrier juvenile laryngeal paralysis and polysneuropathy (AST-JLPP). This project has two objectives. Objective 1 is to conduct a thorough neurologic exam and necropsy of one or more affected American Staffordshire Terrier puppies with the disease The neurologic examination will include characterization of the nature and degree of neurological deficits, as ascertained by a board certified veterinary neurologist. Electromyography (EMG) and various nerve conduction velocities will be measured and recorded. A sedated laryngeal exam will be performed. The necropsy will include both gross and histopathological examination of affected nerves and muscle tissue as well as a standard systemic gross and histopathlogical examination. A summary of the clinical findings and necropsy results will be incorporated into a case report and submitted for publication in the scientific veterinary journal so that veterinarians around the world will be able to recognize and diagnose this novel disease. Objective 2 is to identify the molecular genetic cause for the disease. To accomplish this, we will use Illumina short-read sequencing technology to generate approximately 30-fold average coverage whole genome sequences for two puppies with AST-JLPP. In addition, we will use a genome-wide association study to map the AST-JLPP. Successful discovery of the causal mutation would provide a basis for DNA tests that could be used to confirm a diagnosis of AST-JLPP and also be used for marker-based breeding strategies to avoid affected puppies.
Funding for the research is provided through the collaborative efforts and generosity of Staffordshire Terrier Club of America. The AKC Canine Health Foundation supports the funding of this effort and will oversee administration of funds and scientific progress reports.
None at this time.
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