02669-A: Lipid Composition and Lipid Droplet Dynamics in Canine Pyometra Affected Endometria

Grant Status: Open

Grant Amount: $13,608
Cordula Gabriel, PhD; University of Veterinary Medicine of Vienna
October 1, 2019 - March 31, 2021

Sponsor(s):

Breed(s): -All Dogs
Research Program Area: Reproductive Conditions
Donate to Support this Research Program Area

Abstract

Pyometra is the most common uterine disease in intact bitches leading to potentially life-threatening complications. Escherichia coli (E.coli) are the most abundant isolated pathogens causing pyometra. Previous studies identified increased amounts of lipid droplets (LDs) in canine endometrial epithelial cells (cEECs) occurring in metestrus, the cyclic stage with the most common presence of pyometra. A specialized receptor relevant for lipid-uptake (SR-B1) to be expressed in cEECs and up-regulated in pyometra affected uteri was also identified. Lipids are attractive targets for pathogens to modulate host cell processes in order to allow pathogens’ survival and replication. A correlation of LD accumulation in cEECs with pyometra-related pathogenic E. coli infection is assumed. In this study, the lipid composition in the LDs and different members of LD-coating proteins of the cEECs will be investigated in healthy metestrous and pyometra affected uteri. Furthermore, the effects of bacterial infection on lipid composition and LD formation and function will be investigated. Understanding the regulation of lipid metabolism in pyometra etiology has important implications for exploring new therapeutic strategies for management and treatment of this serious uterine disease in intact bitches.

Publication(s)

None at this time.

Help Future Generations of Dogs

Participate in canine health research by providing samples or by enrolling in a clinical trial. Samples are needed from healthy dogs and dogs affected by specific diseases.

Learn How to Help

Connect With Us:
Get Canine Health News:
Please leave this field empty

© 2018 AKC Canine Health Foundation | Privacy Policy | Site Map

Site by Blackbaud, Inc.

Powered by Blackbaud
nonprofit software