01490-A: Evaluation of Aurora kinase inhibitors in canine lymphoma in vitro

Grant Status: Closed

Grant Amount: $11,538.73
Dr. Keijiro Shiomitsu, D.V.M., Louisiana State University
June 1, 2010 - May 31, 2011
Sponsor(s): American German Shepherd Dog Charitable Foundation, Inc., Bernese Mountain Dog Club of America, English Setter Association of America, Inc., Golden Retriever Foundation
Breed(s): -All Dogs
Research Program Area: Treatment

Project Summary

Aurora kinases are a very attractive target because of their central role in the regulation of the cell cycle. A number of small-molecule aurora kinase inhibitors have been intensively investigated, and many clinical trials with different aurora kinases inhibitors have been conducted in human medicine. However, there are no published data showing the effect of aurora kinase inhibitors in veterinary medicine. The investigators have successfully detected the protein expression of aurora kinase -A and -B in canine tissue and cells. They also evaluated the mRNA expression of aurora-A and -B in GL-1 and EMA cells to examine the relationship between mRNA and protein expression, and they observed that there is a correlation between protein expression and mRNA expression in canine tissue and cells. In the present study, the investigators evaluated the effect of ZM447439 in canine lymphoma cell lines. Cytotoxic effect of aurora kinase inhibitors was seen in canine malignant cells. Distribution and population change of the cell cycle phase caused by the aurora kinase inhibitors was seen using cell cycle analysis. Finally, apoptosis as the mechanism of cell death was observed. These results indicate that aurora kinase inhibitors would be a very attractive therapeutic agent for canine lymphoma, and further in vivo study is warranted.

Publication(s)

- Manuscript in preparation "Evaluation of aurora kinase inhibitor, ZM44 1 7439, in canine lymphoma in vitro" (5-2011)

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