01244-A: DRB, DQA, and DQB Gene Sequencing and Allele Determination in the Pug Dog

Grant Status: Closed

Grant Amount: $12,960
Kimberly A Greer, PhD; Indiana University East
May 1, 2009 - October 31, 2010

Sponsor(s): Pug Dog Club of America, Inc.

Breed(s): Pug
Research Program Area: Neurology
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Project Summary

Our studies of Pug Dog Encephalitis (PDE) identified the genetic components significantly contributing to the development of Pug Dog Encephalitis, including three genes within the dog leukocyte antigen (DLA II) region.: DLA-DRB1, DLA-DQB1, DLA-DQA1. We have completed sequencing the genes and five DRB1 alleles were identified with the most predominant type present in 75.7% of the affected animals. Three DQA1 alleles were identified in the Pug population and the predominant allele was also high among the NME affected dogs. Lastly, the predominant allele of five for DQB1 was present in 75.7% of the affected animals as well, giving us a combined susceptibility haplotype for the dogs. Of the six haplotypes recognized in the Pug Dog, the highest disease associated haplotype was determined. Two of the haplotypes were more likely associated with normal dogs. In sum, we have now identified which genes the dogs more likely to get NME possess and which genes the dogs less likely to get NME possess. Although this gene association is not 100% predictable, we can sequence the alleles for disease and offer genetic testing. Animals having the most susceptible genotype can begin to be removed from the breeding population and matings can be designed to reduce the overall number of Pugs produced with the NME susceptible genotype.

Publication(s)

Greer, K. A., Wong, A. K., Liu, H., Famula, T. R., Pedersen, N. C., Ruhe, A., … Neff, M. W. (2010). Necrotizing meningoencephalitis of Pug Dogs associates with dog leukocyte antigen class II and resembles acute variant forms of multiple sclerosis. Tissue Antigens. https://doi.org/10.1111/j.1399-0039.2010.01484.x
 
Pedersen, N., Liu, H., Millon, L., & Greer, K. (2011). Dog Leukocyte Antigen Class II–Associated Genetic Risk Testing for Immune Disorders of Dogs: Simplified Approaches Using Pug Dog Necrotizing Meningoencephalitis as a Model. Journal of Veterinary Diagnostic Investigation, 23(1), 68–76. https://doi.org/10.1177/104063871102300110

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