Hypothyroidism (low thyroid function) is the most common endocrine disorder in dogs. It can be effectively treated with synthetic thyroid replacement once it is diagnosed. But accurate diagnosis of hypothyroidism is difficult with the current blood tests available. This project sought to develop an improved blood test for canine hypothyroidism by accurately measuring a hormone called TSH. Researchers isolated the gene responsible for making TSH and used a variety of approaches to make reagents (antibodies) that can specifically react with canine TSH. Unfortunately, they were not able to develop an antibody for the TSH hormone and without this antibody an improved diagnostic test for canine hypothyroidism could not be developed as a result of this research.