The purpose of this study is to identify different types of normal B cells in the canine immune system and therefore improve the ability to study B cell diseases in dogs. One aim of this study is to sequence different populations of B cells based on their location in the lymph node and spleen. The second aim is to sequence B cells in three different disorders: 1) a disease in English Bulldogs that causes non-neoplastic B cell expansion; 2) an aggressive form of small cell B cell lymphoma; 3) diffuse large B cell lymphoma, the most common form of lymphoma in dogs.

The investigators will compare gene expression changes in these B cell diseases to normal B cell subsets. This work will help to determine which type of B cell is affected in these disease states and which mechanisms are affected. By determining the mechanisms that drive disease, we can identify potential therapeutic targets to improve the treatment of these diseases. The goal of this work is to advance our knowledge of the immune system in dogs and improve the understanding of three canine B cell diseases.