Canine lung cancer is a devastating disease with minimal progress over the last 20 years. One of the important aspects of staging in dogs with lung cancer is the assessment for lymph node spread. Due to the small size of the lung-associated lymph nodes, these lymph nodes are often not removed at the time of surgery. Specialized techniques that allow for visualization of the lymph nodes before and during surgery can improve treatment methods and the surgical experience, ultimately improving patient treatment and outcomes. The primary objective of this study is to pilot a dual-modality approach to lung sentinel lymph node mapping using computed tomography lymphography (CTL) and indocyanine green (ICG) fluorescence-guided visualization. This novel approach may decrease complications after surgery and provide the most accurate information about metastasis of lung tumors in canine patients.