Glioblastoma multiforme (GBM), a tumor of the brain, is one of the most common and most malignant in the dog. It is found primarily in the Boxer with a 30 percent incidence. The use of modern technologies such as MRI and CT scan in veterinary practice facilitated the detection of brain tumors in dogs. Consequently, the requests by pet owners for biopsy, surgical removal and therapy of brain tumors has greatly increased in recent years. Therefore, there is a need to improve our understanding of glioma development and biological behavior that will allow us to develop new therapeutic modalities. Recent evidence suggest that GBM develop from specific, multipotential stem cells located in the brain subventricular region (SVZ). We propose herein to use a novel approach aimed at understanding the cellular origin of GBMs by using specific biological markers in in vitro and in vivo experiments. In addition, we will use specific growth factors to manipulate the cellular growth and differentiation of these stem cells that can be used as a novel therapeutic paradigm for the treatment of GBMs.