Chagas disease is a parasitic infection that can cause acute death and chronic heart disease in any breed of dog, and there is no vaccination or approved treatment. There is currently an unprecedented recognition for canine Chagas disease in the southern US, where infected kissing bug vectors occur. The disease is well-studied in Latin America, but the current lack of knowledge about canine infection in the US hinders our ability to protect canine health. Infected dogs occur across the range of kissing bugs in the southern half of the US, and Texas is a particular hotspot for infection due to the diversity of kissing bugs and high parasite infection prevalence. The investigators will establish a network of AKC breeding kennels in 4 key areas representing range limits of different vector species to: (1) characterize heart abnormalities of infected dogs using ECG and cardiac troponin I, a non-invasive biomarker of cardiac injury; (2) collect kissing bugs from kennel environments using complementary methods including a trained bug scent detection dog to determine vector infection prevalence and blood meal sources; and (3) characterize parasite strains in dogs and vectors because different genetic variants of the parasite are associated with different disease outcomes. Importantly, because this zoonotic disease is an emerging public health threat to canine owners and the veterinary community, the discoveries made will help to simultaneously advance both canine and human health initiatives.