02118-A: Targeting the Mechanism of Bacterial Adherence during Pyometra to Develop an Effective, Non-Invasive Treatment for Disease

Grant Status: Closed

Grant Amount: $10,368
Dr. Cordula Bartel, PhD, University of Veterinary Medicine of Vienna
July 1, 2014 - November 30, 2015
Sponsor(s): American Cavalier King Charles Spaniel Club Charitable Trust, American Miniature Schnauzer Club, Bernese Mountain Dog Club of America, Boston Terrier Club of America, Chihuahua Club of America, Chow Chow Club, Inc., Collie Health Foundation, English Setter Association of America, Inc., Golden Retriever Foundation, Mastiff Club of America Charitable Trust, Pembroke Welsh Corgi Club of America, Pug Dog Club of America, Inc., Samoyed Club of America Education & Research Foundation, Siberian Husky Club of America, Inc., Standard Schnauzer Club of America, Tibetan Spaniel Club of America
Breed(s): -All Dogs
Research Program Area: Reproductive Conditions and Disease

Project Summary

immunohistochemistry. Therefore, the increased levels of SR-B1 in pyometra affected uteri indicate a potential role of this scavenger receptor in endometrial bacterial adhesion.
Follow-up studies will be performed in the laboratory with cell culture experiments (in vitro) using uterine tissues obtained from routine ovariohysterectomies. A cell culture model of the canine endometrial surface epithelium will help to investigate the influence of hormones, cytokines and bacteria on SR-B1 expression and allows the experimental blocking of SR-B1 without animal testing. In the follow-up study we hypothesize that blocking of SR-B1 in vitro will lead to decreased bacterial binding and inflammatory response via cytokine release as it was observed in other pathological conditions in human diseases and murine models. If these studies are successful we suggest flushing of the canine uterus with a SR-B1 blocking solution as a preventative procedure in exposed bitches or as additional treatment in conservative pyometra therapy.

Publication(s)

Gabriel, C., Becher-Deichsel, A., Hlavaty, J., Mair, G., & Walter, I. (2016). The physiological expression of scavenger receptor SR-B1 in canine endometrial and placental epithelial cells and its potential involvement in pathogenesis of pyometra. Theriogenology, 85(9), 1599–1609.e2. http://dx.doi.org/10.1016/j.theriogenology.2016.01.021

Mair G, Unger H, Gabriel C. Combining RPL27 with OAZ1 or RPL8 as Universal Normalizers of Gene Expression in Canine Endometrial Studies. Int J Vet Sci Technol. 2017;1(1): 023-034. http://www.scireslit.com/Veterinary/IJVST-ID15.pdf

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