403: Application of the 7.5X Canine Genome Assembly to Generate a 1Mb Cytogenetic BAC-Map of the Canine Genome

Grant Status: Closed

Grant Amount: $135,816
Matthew Breen, PhD; North Carolina State University
January 1, 2006 - December 31, 2007

Sponsor(s):

Breed(s): -All Dogs
Research Program Area: General Canine Health
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Abstract

Many cancers are associated with characteristic chromosome aberrations that have diagnostic and prognostic significance. The identification of chromosome aberrations now plays a key role in defining many cancers and in selecting appropriate therapy. In a previous AKC CHF funded study, we developed a form of genome screening for canine cancers, referred to as canine array-CGH. This approach allows us to identify chromosome aberrations much more quickly and with greater accuracy than was previously possible. With the recent sequencing of the canine genome, we are now presented with the resources to develop a genome-wide array that will allow us to identify chromosome aberrations at a resolution previously only available to human cancer researchers. In this proposal we will generate an array that assesses the status of the canine genome at each of 3,000 evenly spaced points. By using reagents that are part of the canine genome assembly, we will be able to translate tumor chromosome aberrations directly into canine DNA sequence data in order to identify the genes that are involved. Once developed we will use the array to assess the chromosome status of a series of canine brain tumors, as a means to identify the genes involved in this devastating cancer.

Publication(s)

Thomas, R., Duke, S. E., Karlsson, E. K., Evans, A., Ellis, P., Lindblad-Toh, K., … Breen, M. (2008). A genome assembly-integrated dog 1 Mb BAC microarray: a cytogenetic resource for canine cancer studies and comparative genomic analysis. Cytogenetic and Genome Research, 122(2), 110–121. https://doi.org/10.1159/000163088
 
Thomas, Rachael, Duke, S. E., Bloom, S. K., Breen, T. E., Young, A. C., Feiste, E., … Breen, M. (2007). A Cytogenetically Characterized, Genome-Anchored 10-Mb BAC Set and CGH Array for the Domestic Dog. Journal of Heredity, 98(5), 474–484. https://doi.org/10.1093/jhered/esm053
 
Thomas, Rachael, Duke, S. E., Wang, H. J., Breen, T. E., Higgins, R. J., Linder, K. E., … Breen, M. (2009). ‘Putting our heads together’: insights into genomic conservation between human and canine intracranial tumors. Journal of Neuro-Oncology, 94(3), 333–349. https://doi.org/10.1007/s11060-009-9877-5

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