Identifying Early Stage Ultra-rare Mutations as Predictive Biomarkers of Lymphoma in High-risk versus Low-risk Breeds Within the Dog Aging Project
The most common type of cancer in dogs is lymphoma, with ~80,000 cases diagnosed annually in the US. Breeds vary considerably in their risk of lymphoma, but it is unclear what accounts for this variation. Cancer typically arises from the accumulation of non-inherited (i.e., somatic) mutations. The purpose of this study is to test the hypothesis that breeds at high risk of lymphoma have higher frequencies of precancerous somatic mutations in white blood cells. This study uses a new technology that makes it possible to detect extremely rare mutations in very small samples of blood with high accuracy. This method represents a >10,000-fold improvement in accuracy over previous methods, and so makes our study possible. By determining if mutation frequency in blood of healthy high-risk and low-risk companion dogs can predict lymphoma risk, this work could lead to the development of novel tests for the early diagnosis and prognosis of canine lymphoma.
The study seeks healthy dogs free of any cancer, with the goal of better understanding canine lymphoma.
Six breeds are included: Boxers, Chow Chows, Dalmatians, Greyhounds, Labrador Retrievers, Rottweilers
Healthy dogs of any age and sex
Willingness to provide blood sample from dog at veterinary clinic
Owners will be asked to bring their dog to a veterinary clinic for the purpose of collecting a blood sample. The sample will be picked up in clinic by a lab technician from the University of Washington immediately following the blood draw.
Owners will be asked to provide an electronic medical record from their veterinarian to confirm the current health status of the dog.
- Dog living in King County, Washington.
- Dog can present to any clinic willing to participate.
- Samples could be sent from other clinics if PBMCs are first isolated and frozen.
Name: Daniel Promislow
Phone: 206 616-6994
Help Future Generations of Dogs
Participate in canine health research by providing samples or by enrolling in a clinical trial. Samples are needed from healthy dogs and dogs affected by specific diseases.