Dogs with Colorectal Cancers, Mammary Cancers, or Head and Neck Squamous Cell Carcinomas
CHF-funded researcher Dr. Shaying Zhao and her team at the University of Georgia are requesting fresh-frozen tumor samples and their matching normal samples (normal tissues adjacent to the tumor, or blood or other normal tissues from the same dog) from dogs developing spontaneous colorectal cancers, mammary cancers, or head and neck squamous cell carcinomas. They also request available case information from the sample supplier, including dog breed, age, sex, anticancer treatment history, tumor site, stage (if available), etc.
Once the samples are received, we will conduct cryosectioning and H&E staining to confirm the histopathological information provided, and perform cryo-microdissection to enrich tumor cells or normal epithelial cells. Genomic DNA and RNA will then be extracted from the dissected tissues. Whole genome sequencing, RNA-seq and other analyses will be performed to uncover genes altered in the canine tumors (1,2). Finally, we will compare the canine findings to those of the corresponding human cancer, particularly from the Cancer Genome Atlas project (cancergenome.nih.gov/), for driver gene discovery for each of three cancer types as described(3).
Please contact Shaying Zhao (firstname.lastname@example.org) for more information on the study and for detailed information on sample requirement, handling, storage and shipping.
1. Tang, J. et al. Copy number abnormalities in sporadic canine colorectal cancers. Genome research 20, 341-350, doi:10.1101/gr.092726.109 (2010).
2. Youmans, L. et al. Frequent alteration of the tumor suppressor gene APC in sporadic canine colorectal tumors. PloS one 7, e50813, doi:10.1371/journal.pone.0050813 (2012).
3. Tang, J. et al. Cancer driver-passenger distinction via sporadic human and dog cancer comparison: a proof-of-principle study with colorectal cancer. Oncogene, doi:10.1038/onc.2013.17 (2013).
Help Future Generations of Dogs
Participate in canine health research by providing samples or by enrolling in a clinical trial. Samples are needed from healthy dogs and dogs affected by specific diseases.