Grant Amount: $54,600

Dr. Gary S. Johnson, DVM PhD, University of Missouri, Columbia

May 22, 1996 - May 21, 1998

Sponsor(s): Alaskan Malamute Research Foundation, Inc., Buckeye Keeshond Club, Keeshond Club of America

Breed(s): -All Dogs

Disease(s): Genome Mapping

Project Summary

This project sought to identify candidate genes for specific diseases in dogs. Candidate genes are genes known to cause specific diseases in other species. The researchers searched for canine equivalents of three human chromosomes. They identified more than 25 Type I markers (markers that are associated with specific genes). These markers, mapped on the canine genome linkage map, provide valuable information to other scientists looking for the genes causing various heritable diseases in dogs.

Publication(s)

- Chen YW, Bermudez AJ, Liu PC, Nonneman D, Johnson GS. A DraIII PCR/RFLP in an intron of the emu lysosomal protective protein gene. Anim Genet. 1998 Oct;29(5):404-5. PubMed PMID: 9800337. - Zhou T, Shibuya H, Liu PC, O'Brien DP, Johnson GS. A NlaIII PCR/RFLP in an intron of the retinitis pigmentosa GTPase regulator gene (RPGR) on the canine X chromosome. Anim Genet. 1998 Aug;29(4):330-1. PubMed PMID: 9745684. - Liu PC, Shibuya H, Nonneman D, Katz ML, Johnson GS. A BseRI PCR/RFLP in an intron of the canine phenol sulfotransferase gene. Anim Genet. 1998 Aug;29(4):329. PubMed PMID: 9745682. - Guo Q, Shibuya H, Nonneman D, Liu PC, Huang TH, Johnson GS. A polymorphic (CA)n microsatellite in the canine lecithin:cholesterol acyltransferase gene. Anim Genet. 1998 Aug;29(4):328-9. PubMed PMID: 9745681. - Stoy SJ, Shibuya H, O'Brien D, Johnson GS. A BsII PCR/RFLP in the renin binding protein (RnBP) gene on canine chromosome X. Anim Genet. 1998 Aug;29(4):327-8. PubMed PMID: 9745680. - Liu PC, Chen YW, Shibuya H, Katz ML, Lubahn DB, Johnson GS. Length polymorphism in a CT-rich microsatellite in an intron of the canine tyrosinase-related protein-2 gene. Anim Genet. 1998 Aug;29(4):327. PubMed PMID: 9745679. - Liu PC, Chen YW, Shibuya H, Lubahn DB, Johnson GS. A length polymorphism in an intron of the canine polycystic kidney disease 1 gene. Anim Genet. 1998 Aug;29(4):322-3. PubMed PMID: 9745674. - Khan S, Shibuya H, Nonneman D, Liu PC, Huang TH, Johnson GS. A polymorphic (TG)n microsatellite in an intron of the canine tyrosine transaminase gene. Anim Genet. 1998 Aug;29(4):322. PubMed PMID: 9745673. - Chen YW, Liu PC, Shibuya H, O'Brien DP, Lubahn DB, Johnson GS. Length polymorphism in a CAG-rich coding region of the canine dentatorubro-pallidoluysian atrophy (DRPLA) gene. Anim Genet. 1998 Jun;29(3):241. PubMed PMID: 9720192. - Ganjam KK, Shibuya H, Stoy SJ, Liu PC, Ganjam VK, Katz ML, Johnson GS. A BstUI PCR/RFLP marker in an intron of the canine fibrillin 1 gene. Anim Genet. 1998 Jun;29(3):240-1. PubMed PMID: 9720191. - Shibuya H, Liu PC, O'Brien DP, Chen YW, Johnson GS. A BbvI mismatch PCR/RFLP marker for the canine huntingtin gene. Anim Genet. 1998 Jun;29(3):239-40. PubMed PMID: 9720189. - Zhou T, Nonneman D, Shibuya H, Khan S, Liu PC, Johnson GS. An AvaII PCR/RFLP in an exon of the canine gene for the beta subunit of the amiloride-sensitive sodium channel (SCNN1B). Anim Genet. 1998 Jun;29(3):239. PubMed PMID: 9720188. - Liu PC, Chen YW, Shibuya H, Katz ML, Lubahn DB, Johnson GS. A polymorphic (GA)n microsatellite in an intron of the canine endothelin-B receptor gene. Anim Genet. 1998 Jun;29(3):236. PubMed PMID: 9720185. - Shibuya H, Liu PC, Katz ML, Siakotos AN, Nonneman DJ, Johnson GS. Coding sequence and exon/intron organization of the canine CLN3 (Batten disease) gene and its exclusion as the locus for ceroid-lipofuscinosis in English setter dogs. J Neurosci Res. 1998 May 1;52(3):268-75. PubMed PMID: 9590435. - Liu PC, Shibuya H, Lubahn DB, Johnson GS. A polymorphic (CT)n microsatellite in an intron of the canine SA-hypertension gene. Anim Genet. 1998 Feb;29(1):72. PubMed PMID: 9682465. - Shibuya H, Cassells MW, Huang TH, Johnson GS. A polymorphic (TG)n microsatellite in an intron of the canine angiotensin I converting enzyme gene. Anim Genet. 1998 Feb;29(1):66-7. PubMed PMID: 9682458. - Liu PC, Shibuya H, Katz ML, Johnson GS. A BsmFI PCR/RFLP in the canine CD19 gene. Anim Genet. 1998 Feb;29(1):64-5. PubMed PMID: 9682456.